med1 2  (Radboud University)

Journal: Nature communications

Article Title: MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex

doi: 10.1038/ncomms6941

Figure Lengend Snippet: (a) Diagram of the genes and primer locations used for ChIP. (b) Occupancy of Mediator subunits MED26, MED23 and MED1 at a subset of snRNA genes including RNU1, RNU4, RNU5 and U6, and c-Myc genes. Ct values of each ChIP were normalized to that of input. Data points are the average of three independent experiments, and error bars show s.d.. The P values for the indicated comparisons were determined by Student’s t test (*, P < 0.05; **, P < 0.01). (c) Colocalization of MED26 (red) with coilin (green) in Cajal bodies. HeLa cells were fixed by methanol and stained with anti-MED26 and anti-Coilin antibodies. Scale bars, 2 µm.

Article Snippet: Anti-FLAG M2 antibodies (1:2000 dilutions, Sigma-Aldrich Corp.), anti-HA antibodies (1:2000 dilutions, Covance, Princeton, NJ), anti-Myc antibodies (1:1000 dilutions, Covance), anti-TAF7 antibodies (1:200 dilutions, sc-101167, Santa Cruz Biothecnology, Santa Cruz, CA), anti-ELL antibodies (1:1000 dilutions, A301-645A, Bethyl Laboratories, Montgomery, TX), anti-EAF2 antibodies (1:1000 dilutions, A302-502A-1, Bethyl Laboratories), anti-Rpb1 antibodies (1:2000 dilutions, sc-899 X, Santa Cruz) and anti-MED1 antibodies (1:2000 dilutions, sc-5334 X, Santa Cruz) were used in Western blots.

Techniques: Staining